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Recently submitted to OmniScience...

Content type: Methods and Protocols

  • Submitted 1 month 2 weeks ago by ktheriault.

    Method Type:

    Human PSC Culture - Before you start

    Human pluripotent stem cells are a field of research that has become very popular relatively recently, mainly due to Shinya Yamanaka's discovery that it is possible to turn fibroblasts into pluripo

    Read more >>
    Pros: 
    Cool Data
    Cons: 
    Tons of money and work
    0
    No votes yet
    Activity

    2 comments

    Viewed 62 times

  • Submitted 1 month 2 weeks ago by ktheriault.

    Method Type:

    Human PSC Culture - Feeder Free with Conditioned Media - Part2: Thawing Stem Cells

    I'm going to skip the usual warning, as hopefully you've seen it in parts 1 and 1.5.  I am going to start this protocol assuming you have already made hPSC media, plated matrigel, and have iME

    Read more >>
    Pros: 
    Works
    Cons: 
    Recovery is somewhat low, but normal for hPSCs
    0
    No votes yet
    Activity

    0 comments

    Viewed 25 times

  • Submitted 1 month 2 weeks ago by ktheriault.

    Method Type:

    Human PSC Culture - Feeder Free with Conditioned Media - Part1.5: Feeder Cells and Surface Coatings

    A few notes: Human ESC/iPSC culture is extremely sensitive and requires flawless sterile technique and attention to detail.  You can NOT treat them the same as HE

    Read more >>
    Pros: 
    With feeders gives very "classic" hPSC morphology, feeder fr
    Cons: 
    Maintenance of extra cell lines, matrigel is tricky to work with
    0
    No votes yet
    Activity

    0 comments

    Viewed 20 times

  • Submitted 1 month 2 weeks ago by ktheriault.

    Method Type:

    Human PSC Culture - Feeder Free with Conditioned Media - Part1: Media

    A few notes: Human ESC/iPSC culture is extremely sensitive and requires flawless sterile technique and attention to detail.  You can NOT treat them the same as HE

    Read more >>
    Pros: 
    Cheapest option, is the "old reliable" of stem cell medias
    Cons: 
    A lot of moving parts, requires MEF conditioning to maintain stemness
    0
    No votes yet
    Activity

    0 comments

    Viewed 30 times

  • Submitted 1 month 3 weeks ago by mbell.

    Method Type:

    Hormone Silicone Capsules

    Your final capsule will be a set length of hormone according to your desired circulating concentration, plus glue on each end.

    Read more >>
    0
    No votes yet
    Activity

    0 comments

    Viewed 28 times

  • Submitted 1 month 3 weeks ago by mbell.

    Method Type:

    cFos Immunohistochemistry

    This protocol uses the following antibodies and reagents that have all been proven to work well: 

    Read more >>
    Pros: 
    this antibody has never given us any problems, and double labels well
    Cons: 
    uses DAB, 48 hour primary incubation
    5
    Average: 5 (1 vote)
    Activity

    0 comments

    Viewed 34 times

Content type: Product Review:

  • Submitted 1 month 2 weeks ago by kgonzales.
    1
    Average: 1 (1 vote)

    Catalogue number:

    Product application:

    • Surgical Tools
    Product Name: 

    Laboratory animal microtattoo system

    I bought this product thinking that it would be a humane way to tag animals without using toe clippings. I was working with neonatal rats and this seemed like it might be worth the cost.

    Read more >>
    Pros: 
    None, it's pretty bad
    Cons: 
    Cost, poorly designed, difficult to clean properly.
    Activity

    0 comments

    Viewed 42 times

  • Submitted 1 month 2 weeks ago by kgonzales.
    5
    Average: 5 (1 vote)

    Catalogue number:

    Product application:

    • ICC
    • chemical
    • serum
    Product Name: 

    normal goat serum for ICC

    This is more of a FYI for those who use normal goat serum. In the past I've seen people spend hundreds of dollars on NGS for ICCs when you could easily just use this stuff at a fraction of the cost.

    Read more >>
    Pros: 
    Cheap! 500ml for $100. Works just as well as more expensive units
    Cons: 
    none
    Activity

    0 comments

    Viewed 29 times

  • Submitted 2 months 23 hours ago by kgonzales.
    5
    Average: 5 (1 vote)

    Catalogue number:

    Product application:

    • pcr
    • genotyping
    Product Name: 

    DirectPCR lysis reagent (mouse tail)

    Really simple! You take some mouse tail (they have other options, including ear, egg yolk, cells etc), you place it in their buffer, incubate for 45 minutes at 85c, and then you run PCR.

    Read more >>
    Pros: 
    Really easy to use and saves a lot of time/money when genotyping a lot of animals
    Cons: 
    None
    Activity

    0 comments

    Viewed 39 times

Content type: Proven to work immuno and westerns

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